Enterovirus belongs to the Picomaviridas family, including poliovirus, Coxsackie virus, Echo virus and new enteroviruses. Human enterovirus is a single-stranded positive-stranded RNA virus, the virus particles are spherical, icosahedral three-dimensional symmetry, without envelope. The genome is about 7000 bp in length, including non-coding regions at the 5' and 3' ends and a large open reading frame (ORF) in the middle. Humans are the only host of human enterovirus, and the infection can lead to a variety of diseases, including paralytic disease, aseptic meningitis, myocardial injury, hand, foot and mouth disease, conjunctivitis, and rash. Patients and hidden infections are the source of infection, and hidden infections are difficult to identify. This kit detects enterovirus nucleic acid RNA by detecting the 5'-untranslated region sequence, which is used for the auxiliary diagnosis of human enterovirus infection. Clinical or laboratory diagnosis uses virus cell culture separation and neutralization test as the main method to detect universal enterovirus.